Reads Demultiplexing with Cutadapt
Introduction
GBS sequencing of Zea mays NAM population.
Dataset description
Illumina multiplex reads from Zea mays. Each sequencing read contains a barcode sequence at 5' that allow to effectively demultiplex them.
Genomic DNA seq
Genome Analyzer IIx System (Illumina, Inc.)
Publication
https://www.pnas.org/doi/full/10.1073/pnas.1413864112
Original Data
SRA Experiment: NCBI - SRP009896
References (NCBI or Ensembl): NCBI - 25775595
Bioinformatic Analysis
1- Analysis Step
Application
Input
Parameters
Adapter Position: Beginning (5' end)
Allowed Errors: 0
Save Unmatched Sequences: False
Include Sample Name: False
File Format: .fastq.gz
Save Counts Table: True
Execution Time
15-20 min
Output
Result files (including a report, two graphs and a tabular txt file).
96 FASTQ files containing the demultiplexed reads.