Enzyme Code Mapping
Enzymes are a specialised class of proteins responsible for catalysing chemical reactions within the cell, this means they accelerate chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products.
Enzymes are classified into seven categories according to the type of reaction catalysed: Oxidoreductases, transferases, hydrolases, lyases, ligases, isomerases, and translocases.
The Enzyme Commission numerical nomenclature classifies enzymes based on the overall reaction catalysed: it does not identify individual enzymes but rather classes of enzymes catalysing similar reactions.
OmicsBox provides EC annotation through the direct GO > EC mapping file available at the Gene Ontology website. This means that only sequences with GO annotations will eventually show also EC number and that the GO annotation accuracy can be made extensive to Enzyme annotations.
The Enzyme codes will be used to Load KEGG pathways.
Run Enzyme Code Mapping
This feature is available under functional analysis → Blast2GO Annotation → Enzyme Code Mapping.
Successful mapping between GOs and Enzyme Code for each query sequence will populate the Enzyme Code column in the Main Sequence Table.
For Enzyme category statistic charts see the Charts and Statistics page of this user manual.
Figure 1: Run GO-Enzyme Code Mapping