Researchers often want to retrieve a functional profile of these significant genes, in order to gain a better understanding of the underlying biological processes.
Functional enrichment analysis is a procedure to identify functions that are over-represented in a set of genes and may have an association with an experimental condition (e.g. phenotype, treatment…). These methods use statistical approaches to identify significantly enriched or depleted groups of genes.
The Functional Analysis Module offers two different statistical tests in order to Functional Enrichment Analysis, the Fisher Exact Test and Gene Set Enrichment Analysis.
Both Fisher's Exact Test and GSEA Enrichment methods need a ranked ID gene list. For a detailed tutorial on how to obtain these lists in each case, please link here.